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recombinant trypsin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher recombinant trypsin
    Recombinant Trypsin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+trypsin/Trypsin+1%3A250%2C+porcine+pancreas/us12607635-606-13-18
    Average 97 stars, based on 1 article reviews
    recombinant trypsin - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Recombinant:

    Article Title: Method of labelling viral particles comprising anionic lipids with fluorescently-labelled negatively-charged polynucleotides in the presence of polyvalent cations
    Article Snippet: .. In experiments where trypsin was added 0.5 μL of a 0.05× stock of recombinant trypsin (TrypLETM Express Enzyme, Thermo-Fisher Scientific) was added to the final sample volume of 20 μL. ..

    Article Title: Umbilical cord-derived adherent stem cells, preparation method therefor, and use thereof
    Article Snippet: .. The animal component-free recombinant trypsin is commercially available, and for example, it may be TrypLETM Select (GIBCO Invitrogen), TrypLETM Express (GIBCO Invitrogen), TrypZeanTM (Sigma Aldrich), or Recombinant Trypsin solutionTM (Biological Industries). ..

    Article Title: Exploring Functionally Enhanced BLP-Trained Macrophage Subpopulations in S. Aureus Infection: Underlying Mechanisms and Therapeutic Significance.
    Article Snippet: .. Then, the cells were washed with DPBS three times and suspended with recombinant trypsin (Gibco, Invitrogen, USA) to collect the cells. ..

    Article Title: The antigen presentation landscape of cytokine-stressed human pancreatic islets
    Article Snippet: The spleen tissue was dissociated into single-cell suspensions by treatment with collagenase type II enzyme (Sigma-C6885), in order to facilitate separation from extracellular matrix and other unwanted tissue-material. .. Aliquots of both non-treated and cytokine-treated islets were removed from cultures at 48 h and stained for CD45 and intracellularly for hormones., Briefly, islets were dispersed with recombinant trypsin (TryPLE Express, ThermoFisher) at 1X for 10 min at 37°C with occasional shaking. .. After washing, islet dispersed cells were stained with stained for viability (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit; Thermo Fisher Scientific), blocked with 50% human sera, and surface stained for CD45-APC-H7 (clone 2D1; BD Biosciences) and anti-HLA-DR-Alexa Fluor 700 (clone L243, BioLegend).

    Article Title: Development of Sustainable, Fiber-Rich and Food-Grade Scaffolds for Cultivated Meat Using Alginate Microcarriers and Plant-Based Oleogel Matrices via 3D Printing
    Article Snippet: Cuprum sulfate, chlorohydric acid, n-hexane, dry methanol, sodium sulfate, potassium sulfate, sodium hydroxide, sodium thiosulfate, boric acid, Sodium alginate (Mw: 12–40 KDa), Calcium Chloride, Ficoll-Paque, glutaraldehyde, sesame oil, and Type I collagen were purchased from Merck Ltd (New Jersey, United States). .. Meanwhile, Tashiro indicator, 5 % phenol solution, sulfuric acid, EMEM, Fetal Bovine Serum, cell culture slides, 0.05 % recombinant trypsin 0.02 % EDTA solution, 1 % Crystal Violet solution, Cytochalasin-B, HEPES 1 M, mitomycin, acridine orange and PBS pH 7.4 were acquired from Thermo Fisher Scientific Inc (Massachusetts, United States). .. NutriStem XF Media. were obtained from Sartorius AG (Göttingen, Germany).

    Article Title: Exploring Functionally Enhanced BLP‐Trained Macrophage Subpopulations in S. Aureus Infection: Underlying Mechanisms and Therapeutic Significance
    Article Snippet: .. Then, the cells were washed with DPBS three times and suspended with recombinant trypsin (Gibco, Invitrogen, USA) to collect the cells. ..

    Article Title: The antigen presentation landscape of cytokine-stressed human pancreatic islets.
    Article Snippet: The spleen tissue was dissociated into single-cell suspensions by treatment with collagenase type II enzyme (Sigma-C6885), in order to facilitate separation from extracellular matrix and other unwanted tissue-material. .. Aliquots of both non-treated and cytokinetreated islets were removed from cultures at 48 h and stained for CD45 and intracellularly for hormones.19,58 Briefly, islets were dispersed with recombinant trypsin (TryPLE Express, ThermoFisher) at 1X for 10 min at 37oC with occasional shaking. .. After washing, islet dispersed cells were stained with stained for viability (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit; Thermo Fisher Scientific), blocked with 50% human sera, and surface stained for CD45-APC-H7 (clone 2D1; BD Biosciences) and anti-HLA-DR-Alexa Fluor 700 (clone L243, BioLegend).

    Article Title: Human placenta-derived endothelial progenitor cells: an animal-free culture system for efficient expansion.
    Article Snippet: .. The tissue fragments were enzymatically digested using a solution containing 10 mg of Type II collagenase (derived from Clostridium histolyticum; 40508ES76, Yeasen Biotechnology, Shanghai, China), 10 mL of EBM-2 basal medium (CC-3156, LONZA, Basel, Switzerland), and 10 mL of recombinant trypsin (obtained from microbial fermentation; A1285901, Thermo Fisher, Massachusetts, USA). ..

    Staining:

    Article Title: The antigen presentation landscape of cytokine-stressed human pancreatic islets
    Article Snippet: The spleen tissue was dissociated into single-cell suspensions by treatment with collagenase type II enzyme (Sigma-C6885), in order to facilitate separation from extracellular matrix and other unwanted tissue-material. .. Aliquots of both non-treated and cytokine-treated islets were removed from cultures at 48 h and stained for CD45 and intracellularly for hormones., Briefly, islets were dispersed with recombinant trypsin (TryPLE Express, ThermoFisher) at 1X for 10 min at 37°C with occasional shaking. .. After washing, islet dispersed cells were stained with stained for viability (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit; Thermo Fisher Scientific), blocked with 50% human sera, and surface stained for CD45-APC-H7 (clone 2D1; BD Biosciences) and anti-HLA-DR-Alexa Fluor 700 (clone L243, BioLegend).

    Article Title: The antigen presentation landscape of cytokine-stressed human pancreatic islets.
    Article Snippet: The spleen tissue was dissociated into single-cell suspensions by treatment with collagenase type II enzyme (Sigma-C6885), in order to facilitate separation from extracellular matrix and other unwanted tissue-material. .. Aliquots of both non-treated and cytokinetreated islets were removed from cultures at 48 h and stained for CD45 and intracellularly for hormones.19,58 Briefly, islets were dispersed with recombinant trypsin (TryPLE Express, ThermoFisher) at 1X for 10 min at 37oC with occasional shaking. .. After washing, islet dispersed cells were stained with stained for viability (LIVE/DEAD Fixable Aqua Dead Cell Stain Kit; Thermo Fisher Scientific), blocked with 50% human sera, and surface stained for CD45-APC-H7 (clone 2D1; BD Biosciences) and anti-HLA-DR-Alexa Fluor 700 (clone L243, BioLegend).

    Cell Culture:

    Article Title: Development of Sustainable, Fiber-Rich and Food-Grade Scaffolds for Cultivated Meat Using Alginate Microcarriers and Plant-Based Oleogel Matrices via 3D Printing
    Article Snippet: Cuprum sulfate, chlorohydric acid, n-hexane, dry methanol, sodium sulfate, potassium sulfate, sodium hydroxide, sodium thiosulfate, boric acid, Sodium alginate (Mw: 12–40 KDa), Calcium Chloride, Ficoll-Paque, glutaraldehyde, sesame oil, and Type I collagen were purchased from Merck Ltd (New Jersey, United States). .. Meanwhile, Tashiro indicator, 5 % phenol solution, sulfuric acid, EMEM, Fetal Bovine Serum, cell culture slides, 0.05 % recombinant trypsin 0.02 % EDTA solution, 1 % Crystal Violet solution, Cytochalasin-B, HEPES 1 M, mitomycin, acridine orange and PBS pH 7.4 were acquired from Thermo Fisher Scientific Inc (Massachusetts, United States). .. NutriStem XF Media. were obtained from Sartorius AG (Göttingen, Germany).

    Derivative Assay:

    Article Title: Human placenta-derived endothelial progenitor cells: an animal-free culture system for efficient expansion.
    Article Snippet: .. The tissue fragments were enzymatically digested using a solution containing 10 mg of Type II collagenase (derived from Clostridium histolyticum; 40508ES76, Yeasen Biotechnology, Shanghai, China), 10 mL of EBM-2 basal medium (CC-3156, LONZA, Basel, Switzerland), and 10 mL of recombinant trypsin (obtained from microbial fermentation; A1285901, Thermo Fisher, Massachusetts, USA). ..



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